Science Practicum Activities through Freshwater Bioremediation Experiments on Biotechnology Materials in Junior High Schools
Abstract
Abstract This research aims to design practical learning for bioremediation of aquatic ecosystems from dye waste by utilizing enzymes extracted from baglog waste. The topic chosen was bioremediation of water polluted by acid blue 113. Purpose of this practicum is to provide in learning science by conducting a practicum that supports environmental sustainability. Variables in this study were enzyme extracts taken from baglog waste of brown oyster mushroom and the percentage of textile dyedecolorization. As a results showed that the percentage of decolorization of wastewater polluted by acid blue dye 113 was as follows concentrations of 30 ppm (45.41 percent), 45 ppm (49.7 percent), 60 ppm (35.08 percent), and 75 ppm (23.09 percent). The enzyme extract of mushroom baglog waste can effectively degrade the content of acid blue 113 textile dye which is dissolved in water. This study is the first to be conducted in Indonesia which can be done easly and using available materials.
Keywords bioremediation, decolorization, baglog waste, mushroom.
Abstrak Penelitian ini bertujuan merancang pembelajaran praktikum tentang bioremediasi ekosistem perairan dari limbah pewarna dengan memanfaatkan enzim yang diekstrak dari limbah baglog. Topik praktikum yang dipilih adalah bioremediasi air yang tercemar pewarna tekstil acid blue 113. Tujuan praktikum ini adalah untuk memberikan bekal dalam pembelajaran IPA dengan melakukan praktikum yang mendukung kelestarian lingkungan. Variabel dalam penelitian ini adalah ekstrak enzim yang diambil dari limbah baglog jamur tiram coklat dan persentase dekolorisasi zat warna tekstil. Hasil penelitian menunjukkan bahwa persentase dekolorisasi air limbah tercemar zat warna asam biru 113 adalah sebagai berikut konsentrasi 30 ppm (45,41 persen), 45 ppm (49,7 persen), 60 ppm (35,08 persen), dan 75 ppm (23,09 persen) ). Hasilnya menunjukkan bahwa ekstrak enzim limbah baglog jamur secara efektif dapat mendegradasi zat pewarna tekstil acid blue 113 yang terlarut dalam air. Studi ini merupakan yang pertama kali dilakukan di Indonesia yang dapat dilakukan dengan mudah dan menggunakan bahan-bahan yang tersedia.
Kata kunci bioremediasi, dekolorisasi, limbah baglog, jamur.
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Beeton, A. M. (2002). Large freshwater lakes: present state, trends, and future. Environmental Conservation, 29(1), 21-38.
Dimawarnita, F., & Perwitasari, U. (2017). Pemanfaatan tandan kosong kelapa sawit untuk produksi jamur tiram (Pleurotus sp.) dan enzim ligninase. Jurnal Mikologi Indonesia, 1(2), 100-108.
Duda, H. J., Susilo, H., & Newcombe, P. (2019). Enhancing different ethnicity science process skills: Problem-based learning through practicum and authentic assessment. International Journal of Instruction, 12(1), 1207-1222.
Fitriani, V. (2019). Analisis Kebutuhan Siswa Terhadap Panduan Praktikum IPA Berbasis Problem Based learning. Journal Of Education In Mathematics, Science, And Technology, 4(1), 10-15.
Gahlout, M., Gupte, S., & Gupte, A. (2013). Optimization of culture condition for enhanced decolorization and degradation of azo dye reactive violet 1 with concomitant production of ligninolytic enzymes by Ganoderma cupreum AG-1. 3 Biotech, 3(2), 143-152.
Gassara, F., Brar, S. K., Tyagi, R., Verma, M., & Surampalli, R. (2010). Screening of agro-industrial wastes to produce ligninolytic enzymes by Phanerochaete chrysosporium. Biochemical Engineering Journal, 49(3), 388-394.
Hofrichter, M. (2002). lignin conversion by manganese peroxidase (MnP). Enzyme and Microbial technology, 30(4), 454-466.
Israilides, C., & Philippoussis, A. (2003). Bio-technologies of recycling agro-industrial wastes for the production of commercially important fungal polysaccharides and mushrooms. Biotechnology and Genetic Engineering Reviews, 20(1), 247-260.
Lellis, B., Fávaro-Polonio, C. Z., Pamphile, J. A., & Polonio, J. C. (2019). Effects of textile dyes on health and the environment and bioremediation potential of living organisms. Biotechnology Research and Innovation, 3(2), 275-290.
Oktafianto, W. R. (2014). Kefektifan Pembelajaran Praktikum Ipa Berbantu Lks Discovery Untuk Mengembangkan Keterampilan Proses Sains. UPEJ Unnes Physics Education Journal, 3(1).
Putri, A. C. (2019). Pengaplikasian Prinsip-Prinsip Green Chemistry dalam Pelaksanaan Pembelajaran Kimia sebagai Pendekatan untuk Pencegahan Pencemaran Akibat Bahan-Bahan Kimia dalam Kegiatan Praktikum di Laboratorium. Journal of Creativity Student, 2(2), 67-73.
Rahayu, S. (2019). Standar Proses PERMENDIKBUD Nomor 65 Tahun 2019.
Rekik, H., Jaouadi, N. Z., Bouacem, K., Zenati, B., Kourdali, S., Badis, A., Annane, R., Bouanane-Darenfed, A., Bejar, S., & Jaouadi, B. (2019). Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization. International journal of biological macromolecules, 125, 514-525.
Sen, S. K., Raut, S., Bandyopadhyay, P., & Raut, S. (2016). Fungal decolouration and degradation of azo dyes: a review. Fungal Biology Reviews, 30(3), 112-133.
Subamia, I. D. P., Wahyuni, I. G. A. N. S., & Widiasih, N. N. (2014). Pengembangan Perangkat Penunjang Praktikum IPA SMP Berbasis Lingkungan. Jurnal Pendidikan dan Pengajaran, 47(1).
Subiantoro, A. W. (2010). Pentingnya praktikum dalam pembelajaran IPA. Yogyakarta: Universitas Negeri Yogyakarta, 7(5).
Suryawati, E., Suzanti, F., Zulfarina, Z., Putriana, A., & Febrianti, L. (2020). The implementation of local environmental problem-based learning student worksheets to strengthen environmental literacy. Jurnal Pendidikan IPA Indonesia, 9(2), 169-178.
Susilowati, S. (2015). Analisis Pedagogical Content Knowledge Guru IPA SMP Kelas VIII dalam Implementasi Kurikulum 2013. Jurnal Pendidikan Matematika dan Sains, 3(1), 72-78.
Tuomela, M., Vikman, M., Hatakka, A., & Itävaara, M. (2000). Biodegradation of lignin in a compost environment: a review. Bioresource technology, 72(2), 169-183.
Vozzo, L., & Smith, P. (2017). Caretakers or Undertakers: How Can Education Support Humanity to Build a Sustainable Future? In Reimagining sustainability in precarious times (pp. 293-304). Springer.
Wahyuni, S., Rahayu, Y. S., & Indana, S. (2020). Development of Problem-Based Learning Student Worksheets to Facilitate Students’ Problem Solving Skills. Jurnal Penelitian Pendidikan IPA, 6(2), 162-165.
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